Molecular detection of Herpes Simplex Type 1 and 2 (HSV1 and HSV2)
The Herpes Simplex Virus (HSV) is a DNA virus, particularly common in the human population. There are two types of Herpes Simplex Virus (HSV):
- type 1 (HSV1), which causes mainly herpes labialis, and
- type 2 (HSV2), which is the main cause of genital herpes.
When the virus HSV infects the body it remains forever and can be activated in cases where there is defect in the immune system. Typical clinical symptoms are the formation of ulcers on the genitals, lips and mouth.
The genetic material (DNA) of the viruses HSV 1 and 2 is detected with specific primers using polymerase chain reaction (PCR) and evaluation of results with agarose gel electrophoresis.
The biological sample used is cervical or endometrial.