Molecular detection of Chlamydia
Chlamydia infection in the reproductive tract is caused by the bacteria Chlamydia trachomatis and is one of the most common sexually transmitted bacterial infections. It is characterized as the “silent disease”, because people who are infected show no symptoms and do not seek treatment. This results to chronic infection in the genital tract, leading to serious complications including chronic inflammatory disease of the reproductive system, infertility and miscarriage.
It affects both women and men, particularly the cervix and fallopian tubes in women and the urethra and epididymis in men.
Methoology: The genetic material (DNA) of the bacteria Chlamydia trachomatis is detected with specific primers using polymerase chain reaction (PCR) and evaluation of results with agarose gel electrophoresis.
The biological sample used is cervical or endometrial.